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Magnetic lateral flow immunoassay test strip development – Considerations for proof of concept evaluation*

Posted by on in 2017
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Connolly R1O' Kennedy R2. 2017. Methods. 116:132-140. doi: 10.1016/j.ymeth.2017.02.002. Epub 2017 Feb 16.

School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland.
School of Biotechnology, Dublin City University, Glasnevin, Dublin 9, Ireland. Electronic address:


Lateral flow immunoassays (LFIA) have grown to become the predominant test device format for the diagnostics and point-of-care industries. The demand for robust and reproducible LFIAs has been facilitated through scale-up production methods using specialized and automated instruments. However, the feasibility of a LFIA device can still be evaluated in a small-scale laboratory setting through controlled manual preparation methods. The advent of super-paramagnetic (SPMP) labels for use in lateral flow has heralded the possibility of highly sensitive and stable LFIAs. The methods used for the preparation of a magnetic LFIA prototype device using a reserved suite of laboratory equipment are described.

* Normal Chicken IgY antibodies used in this publication were produced by Gallus Immunotech Inc.


Lateral flow immunoassay; super-paramagnetic particles

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