- Published: Wednesday, 04 June 2014 16:34
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Let us label your affinity-purified IgY for you. The most popular labels are horseradish peroxidase (HRP), fluorescein isothiocyanate (FITC), biotin and agarose — the costs are listed below. Please contact us to provide a quote for another label.
Eggs may be kept in the fridge for up to 3 months easily and, possibly for up to 6 months . We would ask that you separate the yolks from the white and pool the yolks that are to be purified BUT PLEASE DO NOT FREEZE THE YOLKS! Adding 0.05% sodium azide (125 ul 10% sodium azide/25 ml egg yolk) will preserve the yolks so they can be shipped at room temperature. Otherwise, they should be shipped on ice. Please ship the yolks the day they are cracked to:
Gallus Immunotech Inc.
6570 First Line West Garafraxa
R.R. #3, Fergus, Ontario
If shipping yolks from outside Canada, please contact us or fax us (519-843-2928) to request an electronic letter to be included with your yolk shipment to expedite custom clearance into Canada.
Included in the cost of purification will be the determination of IgY concentration, vial labeling and a product specification. We are usually able to return your purified IgY to you within a week of receipt of your yolks.
If you will be requiring our IgY purification services on a regular basis or for a bulk order, please inquire about our wholesale prices.
We also purify IgY from quail, goose, duck or other avian eggs. Please ask us for a quote.
Compared to the competition:
The antibody purification kit comes with detailed instructions. Furthermore, should you have problems with your kit, we provide technical support on the antibody purification protocol. More about our our IgY antibody purification kit...
IgY, purified from chicken egg yolk and IgG present in mammalian sera contain antibody specificities that are present in the animal's circulation at the time the eggs were formed or at the time the animal was bled. The IgY that you receive from our standard protocol is approximately 90% pure IgY, containing all the IgY specificities that are present in the hen's circulation and transferred to her egg.
The specific activity (percentage of immunoglobulins that is directed against your antigen) of this IgY fraction (or animal serum) depends on the immunogenicity of your antigen and the genetic make-up of the immunized animal. It is reasonable to expect between 2 and 10% specific activity of IgY/IgG from an animal immunized with a protein greater than 50 kilodaltons. Expect less than 1% specific activity to a peptide from an animal immunized with peptide KLH (there will be a much higher specific activity directed to the carrier KLH portion of the immunogen). For many applications, this non-affinity purified antibodiy fraction will work just fine and it is unnecessary to further purify your IgY/IgG. In fact, further purification may result in the loss of your highest avidity antibodies.
However, sometimes, it is necessary to remove the antibodies that are not antigen specific and this is done by making an antigen column to which the whole IgY/IgG fraction is applied. The IgY/IgG you receive is the antigen-specific fraction that is eluted from the column. To make the affinity column, we will need 5 mg protein antigen (in addition to that supplied for immunizations) at a concentration greater than 3 mg/ml.
Gallus Immunotech Inc. specializes in the development and purification of IgY antibodies, primarily in chickens. However, if you have animal sera from which you would like affinity purified antibodies, we would be happy to do this for you.
If you want affinity-purified IgY from 2 hens immunized with a peptide that we synthesize and conjugate to KLH, please see our Affinity Purifed anti-Peptide Combo for special pricing.
Our custom affinity purification service includes:
How much antigen should you ship us?
We recommend immunization with, between 0.5 and 2 milligrams of purified or recombinant protein per hen and prefer a concentration of at least 2 mg/ml. If possible, please supply us with 4 vials of immunogen (for 4 immunizations), the first vial (for the primary immunization) with twice as much antigen as the others. If you would like your IgY affinity purified, we need an additional 5 mg protein at a concentration greater than 3 mg/ml to make an affinity column.
Where do you ship your antigen?
Ship your purified immunogen, recombinant protein or peptide conjugate to us at:
Exalpha Biologicals, Inc.
2 Shaker Road Unit B101
As soon as we start the immunization, we will e-mail you an immunization/production schedule so you'll know exactly when you can expect your custom IgY.
Do you want us to make your peptide(s)?
Please have a look at our Peptide synthesis page and fill out the custom peptide order form. You may order your custom IgY production on-line or by faxing us the order form. Approximately 3 to 4 weeks after you place your peptide order, we will be contacting you by e-mail with your immunization/production schedule.
Do you want us to Affinity Purify your Custom IgY?
Please have a look at our Affinity Purification page
The cost includes immunizations, animal care and IgY purification from immune and pre-immune eggs.
We will be happy to modify our immunization schedule to meet your needs:
*This price is for pooled IgY antibody purifications. We can provide separate IgY antibody purifications on request.
A single hen, if she is in a typical laying period will produce between 1.5 and 3 grams IgY every month. Within 14 days of receipt of immune IgY, our customer has a choice to purchase additional grams of immune IgY:
If you want to purchase more than 1 gram IgY, or perhaps you haven't decided whether you want to purchase more IgY, or maybe you just want the option in the future to boost your hen and generate some more IgY, you may board your hen with us: