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Yeast surface display for antibody isolation: library construction, library screening, and affinity maturation.*

Posted by on in 2014
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Van Deventer JA1Wittrup KD. 2014. Methods Mol Biol.1131:151-81. doi: 10.1007/978-1-62703-992-5_10. 1. Department of Chemical Engineering, Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA, USA.


Antibodies play key roles as reagents, diagnostics, and therapeutics in numerous biological and biomedical research settings. Although many antibodies are commercially available, oftentimes, specific applications require the development of antibodies with customized properties. Yeast surface display is a robust, versatile, and quantitative method for generating these antibodies and is accessible to single-investigator laboratories. This protocol details the key aspects of yeast surface display library construction and screening.

*Note The Chicken anti-CMYC used in this publication was produced by Gallus Immunotech Inc.

PMID: 24515465[PubMed - in process]

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