Standing out in the field of IgY Immunotechnology

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Using immunoglobulin Y as an alternative antibody for the detection of hepatitis A virus in frozen liver sections.

Posted by on in 2015
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Bentes GA1Lanzarini NM1Lima LR1Manso PP2da Silva Ados S1Mouta Junior Sda S3Guimarães JR1de Moraes MT3Pelajo-Machado M2Pinto MA1. 2015. Mem Inst Oswaldo Cruz. 110(4):577-9. doi: 10.1590/0074-02760140457. Epub 2015 May 15.

  • 1Laboratório de Desenvolvimento Tecnológico em Virologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
  • 2Laboratório de Patologia, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.
  • 3Laboratório de Virologia Comparada e Ambiental, Instituto Oswaldo Cruz, Rio de Janeiro, RJ, Brasil.

Abstract

An increasing amount of research has been conducted on immunoglobulin Y (IgY) because the use of IgY offers several advantages with respect to diagnostic testing, including its easy accessibility, low cost and translatability to large-scale production, in addition to the fact that it can be ethically produced. In a previous work, immunoglobulin was produced and purified from egg yolks (IgY) reactive to hepatitis A virus (HAV) antigens. In the present work, this anti-HAV-specific IgY was used in an indirect immunofluorescence assay to detect viral antigens in liver biopsies that were obtained from experimentally infected cynomolgus monkeys. Fields that were positive for HAV antigen were detected in liver sections using confocal microscopy. In conclusion, egg yolks from immunised hens may be a reliable source for antibody production, which can be employed for immunological studies.

PMID:
 
25993400
 
[PubMed - in process] 
PMCID:
 
PMC4501425
 
Free PMC Article
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