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Therapeutic potential of anti-IL-1β IgY in guinea pigs with allergic asthma induced by ovalbumin.

Posted by on in 2014
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Wei-Xu H1Qin X2Zhu W3Yuan-Yi C2Li-Feng Z2Zhi-Yong L4Dan H2Xiao-Mu W2Guo-Zhu H5. 2014. Mol Immunol. 58(1):139-49. doi: 10.1016/j.molimm.2013.11.006. Epub 2013 Dec 24.

  • 1Department of Radiation Oncology, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
  • 2Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang 330006, China.
  • 3Department of Hematology, Jiangxi Academy of Medical Science, Nanchang 330006, China.
  • 4Department of Experimental Animal, Institute of Occupational Medicine of Jiangxi, Nanchang 330006, China.
  • 5Institute of Clinical Medical Sciences, Jiangxi Province People's Hospital, Nanchang 330006, China. Electronic address: hgz56@126.com.

Abstract

BACKGROUND:

Interleukin-1 beta (IL-1β) plays pivotal roles in the progression of allergic airway inflammation. This study aims to determine whether the blockade of IL-1β can inhibit airway inflammation in guinea pigs with allergic asthma induced by the inhalation of aerosolized ovalbumin (OVA).

METHODS:

Healthy guinea pigs treated with saline were used as normal controls (group C). The guinea pigs with allergic asthma induced by the inhalation of aerosolized OVA were randomly divided into three groups: (1) the M group containing negative control animals treated with saline; (2) the Z1 group containing animals treated by the inhalation of atomized 0.1% anti-IL-1β immunoglobulin yolk (IgY); and (3) the Z2 group containing positive control animals that were treated with budesonide. The inflammatory cells in the peripheral blood (PB) and bronchoalveolar lavage fluid (BALF) were evaluated using methylene blue and eosin staining. Cytokine concentrations were measured using an enzyme-linked immunosorbent assay. Pulmonary sections were examined using hematoxylin-eosin staining.

RESULTS:

Allergic inflammation and damage to the pulmonary tissues were decreased in the Z1 group compared to the M group. Eosinophils and neutrophils in the PB and BALF were significantly decreased in the Z1 group compared to the M group (P<0.05). Treatment with anti-IL-1β IgY significantly reduced the levels of IL-1β, IL-4, IL-8, IL-13, TNF-α, TGF-β1 and IgE in the BALF (P<0.05).

CONCLUSION:

The inhalation of aerosolized anti-IL-1β IgY inhibits pathological responses in the pulmonary tissues of guinea pigs with allergic asthma. The inhibitory activity may be due to the decrease in the numbers of eosinophils and neutrophils and the reduced levels of inflammatory cytokines and IgE in the PB and BALF.

Copyright © 2013 Elsevier Ltd. All rights reserved.

KEYWORDS:

AHR, Allergic asthma, BALF, Cytokines, Guinea pigs, HE, IL-1 receptor antagonist, IL-13, IL-1ra, IL-1β, IL-4, IL-8, IgE, IgY, Immunoglobulin yolk, Infliximab, Interleukin-1β, OVA, Omalizumab, PB, T helper type 2, TGF-β(1), TNF-α, Th2, airway hyperresponsiveness, anti-IgE antibody, anti-TNF-α antibody, bronchoalveolar lavage fluid, hematoxylin-eosin, immunoglobulin E, immunoglobulin yolk, interleukin-13, interleukin-1beta, interleukin-4, interleukin-8, ovalbumin, peripheral blood, sRaw, sTNFR, soluble TNF-α receptor, specific airway resistance, transforming growth factor-beta 1, tumor necrosis factor-alpha

PMID:
24355520
[PubMed - in process]
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