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The Structure of Natural and Recombinant Antibodies.

Posted by on in 2015
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Ma H1O'Kennedy R2. 2015. Methods Mol Biol. 1348:7-11. doi: 10.1007/978-1-4939-2999-3_2.

  • 1School of Biotechnology and Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland. fay2580@gmail.com.
  • 2School of Biotechnology and Biomedical Diagnostics Institute, Dublin City University, Dublin, Ireland.

Abstract

Immunoglobulins (Ig) isotypes A, D, E, G, and M are glycoproteins which are mainly composed of a "Y"-shaped Ig monomer (~150 kDa), consisting of two light and two heavy chains. Both light and heavy chains contain variable (N-terminal) and constant regions (C-terminal). Each light chain consists of one variable domain and one constant domain, whereas each heavy chain has one variable domain and three constant domains. However, heavy-chain antibodies consisting of only heavy chains and lacking the light chains are found in camelids and cartilaginous fishes. Unlike other immunoglobulins, the heavy chain of avian antibody IgY (~180 kDa) consists of four constant domains. The single-chain variable fragment (scFv; ~25 kDa) of an antibody contains variable regions of antibody heavy and light chains. The fragment antigen-binding (Fab; ~50 kDa) region has the full antibody light chain but the heavy chain is composed of a variable region and one constant domain.

KEYWORDS:

Fab; IgG; IgY; ScFv

PMID:
 
26424258
 
[PubMed - in process]
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