Standing out in the field of IgY Immunotechnology

  • Home
    Home A full collection of all the Research Archive entries.
  • Years
    Years Sort entries by year.
  • Tags
    Tags Displays a list of tags that have been used in the blog.
  • Archives
    Archives Contains a list of research entries that were created previously.

The Purification of Natural and Recombinant Peptide Antibodies by Affinity Chromatographic Strategies.

Posted by on in 2015
  • Font size: Larger Smaller
  • Hits: 929
  • Print

Ma H1O'Kennedy R2. 2015. Methods Mol Biol. 1348:153-65. doi: 10.1007/978-1-4939-2999-3_15.

  • 1School of Biotechnology and Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland. fay2580@gmail.com.
  • 2School of Biotechnology and Biomedical Diagnostics Institute, Dublin City University, Glasnevin, Dublin 9, Ireland.

Abstract

 Affinity chromatographic purification, a very convenient and effective antibody purification strategy, is used to isolate peptide antibodies based on specific binding, i.e., binding of the antibody to a column on which its specific ligand is immobilized with subsequent elution of the purified antibody. In addition, the application of purification methods based on the use of proteins A, G, and L, each of which bind to specific domains on an antibody/fragment, or the use of specific tags (e.g., histidine and biotin) attached to antibodies or antigens are also described.

KEYWORDS:

Affinity chromatographic purification; Antibody–antigen binding; Fab; IgG; IgY; ScFv

PMID:
 
26424271
 
[PubMed - in process]
Last modified on