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Study on development of Vipera lebetina snake anti-venom in chicken egg yolk for passive immunization.

Posted by on in 2015
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Zolfagharian H1Dounighi NM1. 2015. Hum Vaccin Immunother. 11(11):2734-9. doi: 10.4161/21645515.2014.985492. Epub 2015 Feb 20.

  • 1a Department of Human Vaccine and Serum ; Razi Vaccine and Serum Research Institute ; Karaj , Iran.


Chicken egg yolk antibodies against Vipera lebetina venom were evaluated for their antivenom potential. White leghorn hens were immunized with detoxified V. lebetina venom (γ-irradiated venom). The detoxified venom (200 μg) was mixed with an equal volume of complete Freund's adjuvant and was injected intramuscularly into the hens. The antibodies showed high activity (1.6 LD50/mL) in egg yolks after 12 d of venom injection. The eggs were collected after 12 days, and the egg yolks were removed and washed with purified water to remove any contamination with egg whites. The purification was performed using a method described by Maya Devi et al., followed by gel filtration (Sephadex G-50). The purity and molecular weight of antivenom antibodies (IgY) were determined using electrophoresis, and the molecular weight was found to be approximately 185 kDa. The potency of IgY was 6 LD50/mL (mice), i.e., 1 mL of IgY could neutralize 43.8 μg of standard V. lebetina venom). Our results showed that 


IgY; Vipera lebetina; antivenom; chicken egg yolk; passive immunization; snake

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 [Available on 2016-02-20]
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