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Stable production of recombinant chicken antibody in CHO-K1 cell line

Posted by on in 2005
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 Shimamoto T, Nishibori N, Aosasa M, Horiuchi H, Furusawa S, Matsuda H.
Biologicals. 2005 Sep;33(3):169-74.
Laboratory of Immunobiology, Department of Molecular and Applied Bioscience, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8528, Japan.

Abstract

When compared with mammalian IgG, chicken IgY is advantageous in terms of cross-reactivity.

In this study, two plasmids were constructed for expression of recombinant chicken IgY derived from a chicken hybridoma. The first was for expression of the light (L) chain, and the other was for the heavy (H) chain with a histidine (His) tag at the carboxy-terminal. After transfection of recombinant chicken IgY gene into Chinese hamster ovary cells, a transfectant designated HF33 that secreted the specific antibody was selected. HF33 cells produced recombinant IgY with His tag at 10-15 microg/10(6) cells/24 h. On Western blotting analysis, the recombinant IgY was detected as one band for the H chain and two bands for the L chain. The recombinant IgY was successfully purified in a one-step procedure using a nickel-affinity resin.

These results indicate that the present recombinant chicken IgY is useful for further applications.

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