Ivanyi J, Fuensalida E, Lydyard PM
Eur J Immunol 1976 Jan 6:25-30
Rosette-forming cells (RFC) from the peripheral blood of sheep red blood cell (SRBC)-immunized chickens were characterized as B cells which manifest antigen-binding receptors but no antibody secretion. Lymphocytes were pretreated at 0 degrees C with anti-Ig serum, washed, incubated further at various temperatures and harvested for the rosette-forming cells (RFC) assay. Anti-Ig treatment blocked all RFC formation and providing that the cells were maintained at less than 10 degrees C regeneration did not occur up to 6 h. Complete recovery of RFC formation occurred within 10 min at 37 degrees C following treatment with low but not with high concentrations of anti-Ig serum. However, recovery from inhibition by high-dose anti-Ig treatment was achieved by including chicken IgG in the incubation medium. The recovery of receptors was reduced following "sandwich" treatment of lymphocytes. When cells were treated with anti-Ig antibody, washed and exposed to various drug inhibitors of "capping" the regeneration of RFC was prevented; in contrast, inhibitors of protein synthesis were ineffective. The results were interpreted in terms of dissociation of anti-Ig antibodies from the Ig receptor at an early stage of the ligand-induced receptor redistribution process.