Standing out in the field of IgY Immunotechnology

  • Home
    Home A full collection of all the Research Archive entries.
  • Years
    Years Sort entries by year.
  • Tags
    Tags Displays a list of tags that have been used in the blog.
  • Archives
    Archives Contains a list of research entries that were created previously.

Purification of immunoglobulins from chicken sera by thiophilic gel chromatography

Posted by on in 2007
  • Font size: Larger Smaller
  • Hits: 1455
  • Print

Constantinoiu CC, Molloy JB, Jorgensen WK, Coleman GT.
Poult Sci. 2007 Sep;86(9):1910-4.

School of Veterinary Science, University of Queensland, Brisbane, Queensland, 4072, Australia. c.constantinoiu@uq.edu.au

Immunoglobulin Y is different from most of the other immunoglobulins because it does not bind protein A or protein G. Thiophilic gel chromatography has been successfully used to purify IgY from chicken egg yolk, but the technology has not previously been used to purify IgY from serum. In this research note, we describe the optimization of T-gel chromatography for purification of IgY from serum. Data are provided on the recovery and purity of IgY obtained using potassium sulfate buffers of different concentrations. Decreasing the strength of potassium sulfate buffer from 0.5 to 0.3 M did not alter the amount of IgY recovered but increased the purity. Using 0.3 M potassium sulphate, we recovered approximately 63.7% of the serum Ig as almost pure IgY.

Last modified on