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Production of specific IgY Helicobacter pylori recombinant OipA protein and assessment of its inhibitory effects towards attachment of H. pylori to AGS cell line.

Posted by on in 2015
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Borhani K1Mobarez AM1Khabiri AR2Behmanesh M3Khoramabadi N1. 2015. Clin Exp Vaccine Res. 4(2):177-83. doi: 10.7774/cevr.2015.4.2.177. Epub 2015 Jul 29.

  • 1Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
  • 2Diagnosis Biotechnology Units, Pasteur Institute of Iran, Alborz, Iran.
  • 3Department of Genetic, Faculty of Life Sciences, Tarbiat Modares University, Tehran, Iran.

Abstract

PURPOSE:

The common triple therapy for Helicobacter pylori is challenged by the increasing cases of antibiotic resistant infections, raising the need to explore alternative therapies. Oral administration of egg yolk immunoglobulin Y (IgY) has been previously reported as a means of passive immunization therapy for H. pylori infections. In this work,  we investigated the inhibitory effect of IgY on the attachment of H. pylori to AGS cell line.

MATERIALS AND METHODS:

Recombinant OipA was prepared. Hens were immunized with recombinant protein three times. IgY was purified from egg yolks of immunized hens using polyethylene glycol precipitation method. The inhibitory effect of the specific immunoglobulin was evaluated in AGS cell line infected with H. pylori.

RESULTS:

The presence of recombinant OipA (30 kD) was confirmed via sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Immunization of hens was confirmed using enzyme-linked immunosorbent assay. The purified IgY from egg yolks were assessed using SDS-PAGE and confirmed by western blot.

CONCLUSION:

The results showed that IgY-OipA had inhibitory effect on attachment of H. pylori to AGS cell line and may be utilized as a therapeutic or prophylaxis material.

KEYWORDS:

AGS cell line; Co-culture techniques; Helicobacter pylori; IgY; OipA

PMID:
 
26273576
 
[PubMed] 
PMCID:
 
PMC4524902
 
Free PMC Article
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