You ZYang HXin WKang LGao SWang JZhang TWang JL. 2014. Hum Vaccin Immunother. 210(8) 

2321-7. [Epub ahead of print]

Abstract

Botulism in human is a devastating intoxication caused mainly by type A, B, and E botulinum neurotoxins (BoNTs). The most effective treatment of botulism is injection of BoNT antiserum in the first 24 h. In this study, a recombinant C-terminal heavy chain of BoNT/B (BHc) was successfully expressed in E. coli. The soluble BHc was used as an antigen to immunize laying hens for yolk immunoglobulin (IgY) production. The purified IgYagainst BHc subunit, preincubated with the BoNT/B, was predominantly involved in the neutralization of BoNT/B toxicity. Furthermore, both intraperitoneal and intragastric administration of the IgY could protect mice from death caused by injection of toxin at a lethal dose. Our results therefore suggest that anti-BHc IgY directed to the Hc domain is effectively involved in the neutralization of BoNT/B toxin and may be considered as preventive and therapeutic intervention in the case of botulism.

KEYWORDS:

Hc fragment; IgY; botulinum neurotoxin; botulism; passive protection

PMID:
 
24946035
 
[PubMed - as supplied by publisher]