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[Preparation and property of two specific anti-Helicobacter pylori immunoglobulins in egg yolk]. [Article in Chinese]

Posted by on in 2015
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  • Yang T1Yang J2Wang B3Pan X4Li J5Zhu J5Zhou Y5Li M3Cao S6. 2015. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 31(2):251-4.
  • 1Sichuan Provincial Key Laboratory of Animal Disease and Human Health, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014; Sichuan Vac Technology Co. Ltd, Chengdu 610041, China.
  • 2Department of Microbiology, West China College of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041; Department of Microbiology, Hubei University of Medicine, Shiyan 442000, China.
  • 3Department of Microbiology, West China College of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China.
  • 4Department of Microbiology, West China College of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041; Sichuan Vac Technology Co. Ltd, Chengdu 610041, China.
  • 5Sichuan Vac Technology Co. Ltd, Chengdu 610041, China.
  • 6Sichuan Provincial Key Laboratory of Animal Disease and Human Health, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China.

Abstract

Objective To prepare and purify anti-Helicobacter (H.) pylori Sydney Strain 1 (SS1) immunoglobulin Y (IgY) and anti-recombinant Ure I-UreB protein (rIB) IgY and investigate their antibacterial effects in vitro. Methods rIB protein and SS1 cell solution were treated by ultrasonic disruption and used as immunogens to immune Leghorn hens. SS1-IgY and IB-IgY were derived from egg yolk with method of the water dilution (WD) combined with ammonium sulfate precipitations and analyzed by growth inhibition test (GIT) and rapid urease test (RUT). Results Two specific IgY with purity of over 80% and titer over 1:12 800 were prepared successfully. Both SS1-IgY and IB-IgY inhibited the growth of H.pylori and reduced H.pylori urease activity in vitro markedly. Conclusion SS1-IgY and IB-IgY can inhibit the growth of H.pylori and reduce the urease activity in vitro.

PMID:
 
25652869
 
[PubMed - in process]
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