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Passive protection effect of anti-Vibrio anguillarum IgY-encapsulated feed on half-smooth tongue sole (Cynoglossus semilaevi) against V. anguillarum.

Posted by on in 2016
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Gao X1Zhang X2Sun J1Du X3Li X3Zhang Y3Lin L4. 2016. Fish Shellfish Immunol. 56:483-8. doi: 10.1016/j.fsi.2016.07.041. Epub 2016 Aug 2.

1College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; College of Ocean, Huaihai Institute of Technology, Lianyungang, 222005, China.
2College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China. Electronic address: zxj9307@163.com.
3College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
4Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; College of Marine Sciences, South China Normal University, Guangzhou, Guangdong, 510642, China. Electronic address: linli@mail.hzau.edu.cn.

 

Abstract

Vibrio anguillarum is one of the most harmful pathogens associated with hemorrhage septicemia syndrome in the half-smooth tongue sole (C. semilaevis) due to its high virulence. In this study, we attempted to treat half-smooth tongue sole with anti-V. anguillarum egg yolk powder to elicit a passive immunity directly against V. anguillarum infection. Anti-V. anguillarum IgY was β-cyclodextrin encapsulated in egg yolk powders as feed, which could avoid antibody inactivation in the gastrointestinal tract of half-smooth tongue sole. The IgY had an inhibiting effect on the infection of V. anguillarum in vitro. The survival rate of half-smooth tongue sole fed with basal diet containing 15% anti-V. anguillarum egg yolk powder was 70% after 7 days post-V. anguillarum challenge (10(7) CFU), which was significantly higher than those fed without anti-V. anguillarum egg yolk powder. As well, the bacterial burden in blood, liver, spleen and kidney was significantly lower in half-smooth tongue sole fed with specific IgY than those fed with non-specific IgY. These results suggested that pathogen-specific IgY may provide a valuable treatment for vibriosis infection and can be a promising food additive.

KEYWORDS:

Half-smooth tongue sole; Immunoglobulin Y (IgY); Passive immunity; Vibrio anguillarum

PMID:
 
27495124
 
DOI:
 
10.1016/j.fsi.2016.07.041
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