Gao X1, Zhang X2, Sun J1, Du X3, Li X3, Zhang Y3, Lin L4. 2016. Fish Shellfish Immunol. 56:483-8. doi: 10.1016/j.fsi.2016.07.041. Epub 2016 Aug 2.
1College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China; College of Ocean, Huaihai Institute of Technology, Lianyungang, 222005, China.
2College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China. Electronic address: email@example.com.
3College of Animal Science and Technology, Yangzhou University, Yangzhou, 225009, China.
4Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan, 430070, China; College of Marine Sciences, South China Normal University, Guangzhou, Guangdong, 510642, China. Electronic address: firstname.lastname@example.org.
Vibrio anguillarum is one of the most harmful pathogens associated with hemorrhage septicemia syndrome in the half-smooth tongue sole (C. semilaevis) due to its high virulence. In this study, we attempted to treat half-smooth tongue sole with anti-V. anguillarum egg yolk powder to elicit a passive immunity directly against V. anguillarum infection. Anti-V. anguillarum IgY was β-cyclodextrin encapsulated in egg yolk powders as feed, which could avoid antibody inactivation in the gastrointestinal tract of half-smooth tongue sole. The IgY had an inhibiting effect on the infection of V. anguillarum in vitro. The survival rate of half-smooth tongue sole fed with basal diet containing 15% anti-V. anguillarum egg yolk powder was 70% after 7 days post-V. anguillarum challenge (10(7) CFU), which was significantly higher than those fed without anti-V. anguillarum egg yolk powder. As well, the bacterial burden in blood, liver, spleen and kidney was significantly lower in half-smooth tongue sole fed with specific IgY than those fed with non-specific IgY. These results suggested that pathogen-specific IgY may provide a valuable treatment for vibriosis infection and can be a promising food additive.
Copyright © 2016 Elsevier Ltd. All rights reserved.
Half-smooth tongue sole; Immunoglobulin Y (IgY); Passive immunity; Vibrio anguillarum