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Passive Immune-Protection of Litopenaeus vannamei against Vibrio harveyi and Vibrio parahaemolyticus Infections with Anti-Vibrio Egg Yolk (IgY)-Encapsulated Feed.

Posted by on in 2016
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Gao X1,2Zhang X3Lin L4Yao D5Sun J6,7Du X8Li X9Zhang Y102016. Int J Mol Sci.;17(5). pii: E723. doi: 10.3390/ijms17050723.

1
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. gaoxj336@163.com.
2
College of Ocean, Huaihai Institute of Technology, Lianyungang 222005, China. gaoxj336@163.com.
3
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. zxj9307@163.com.
4
Department of Aquatic Animal Medicine, College of Fisheries, Huazhong Agricultural University, Wuhan 430070, China. linli@mail.hzau.edu.cn.
5
Institute of Botany Jiangsu Province and Chinese Academy Sciences, Nanjing 210014, China. yaodongrui@hotmail.com.
6
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. y0741810@126.com.
7
College of Ocean, Huaihai Institute of Technology, Lianyungang 222005, China. y0741810@126.com.
8
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. duxuedi@hotmail.com.
9
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. lixm0302@163.com.
10
College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China. 18861339319@163.com.

 

Abstract

Vibrio spp. are major causes of mortality in white shrimp (Litopenaeus vannamei) which is lacking adaptive immunity. Passive immunization with a specific egg yolk antibody (IgY) is a potential method for the protection of shrimp against vibriosis. In this study, immune effects of the specific egg yolk powders (IgY) against both V. harveyi and V. parahaemolyticus on white shrimp were evaluated. The egg yolk powders against V. harveyi and V. parahaemolyticus for passive immunization of white shrimp were prepared, while a tube agglutination assay and an indirect enzyme-linked immunosorbent assay (ELISA) were used for detection of IgY titer. Anti-Vibrio egg yolk was encapsulated by β-cyclodextrin, which could keep the activity of the antibody in the gastrointestinal tract of shrimp. The results showed that the anti-Vibrio egg powders had an inhibiting effect on V. harveyi and V. parahaemolyticus in vitro. Lower mortality of infected zoeae, mysis, and postlarva was observed in groups fed with anti-Vibrio egg powders, compared with those fed with normal egg powders. The bacterial load in postlarva fed with specific egg powders in seeding ponds was significantly lower than those fed with normal egg powders in seeding ponds. These results show that passive immunization by oral administration with specific egg yolk powders (IgY) may provide a valuable protection of vibrio infections in white shrimp.

KEYWORDS:

IgY; Litopenaeus vannamei; Vibrio harveyi; Vibrio parahaemolyticus; passive immunization

PMID:
 
27196895
 
PMCID:
 
PMC4881545
 
DOI:
 
10.3390/ijms17050723
[Indexed for MEDLINE] 
Free PMC Article
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