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Multiplex enzyme immunoassay system for the simultaneous detection of multiple allergens in foods

Posted by on in 2003
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 Food Control, Volume 14, Issue 1 , January 2003, Pages 43-47
Burton W. Blais, Melissa Gaudreault and Lucille M. Phillippe
Laboratory Services Division, Canadian Food Inspection Agency, Bldg. 22, CEF, Ottawa, Canada K1A 0C6
Received 3 September 2001; revised 17 May 2002; accepted 20 May 2002; Available online 13 November 2002.


An enzyme immunoassay (EIA) system was developed for the simultaneous detection of multiple allergens in foods. This assay utilized a reverse dot blot EIA format in which egg yolk antibodies (IgY) specific for different allergens were immobilized in discrete spots on a strip of polyester cloth, which was then contacted with the test sample. The binding of antigens to a specific spot on the strip was revealed by sequential reactions with a pool of biotinylated allergen-specific antibodies, streptavidin-peroxidase conjugate and a chromogenic substrate. As an example of the applicability of this multiplex EIA system, we demonstrate the detection of three important food allergens: peanut, hazelnut and Brazil nut.

Author Keywords: Multiplex; Enzyme immunoassay; Allergens; Peanut; Hazelnut; Brazil nut

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