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Mapping polyclonal antibody responses to bacterial infection using next generation phage display.

Posted by on in 2016
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Naqid IA1Owen JP2Maddison BC2Spiliotopoulos A1Emes RD1,3Warry A1,3Tch√≥rzewska MA4,5Martelli F4Gosling RJ4Davies RH4La Ragione RM6Gough KC12016. Sci Rep. 6:24232. doi: 10.1038/srep24232.

1School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.
2ADAS UK, School of Veterinary Medicine and Science, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.
3Advanced Data Analysis Centre, The University of Nottingham, Sutton Bonington Campus, College Road, Sutton Bonington, Leicestershire, LE12 5RD, UK.
4Animal and Plant Health Agency, Woodham Lane, New Haw, Addlestone, Surrey. KT15 3NB, UK.
5School of Biological Science, Royal Holloway, University of London, Egham, Surrey. TW20 0EX, UK.
6School of Veterinary Medicine, Daphne Jackson Road, University of Surrey, Guildford, Surrey. GU2 7AL, UK.

 

Abstract

Mapping polyclonal antibody responses to infectious diseases to identify individual epitopes has the potential to underpin the development of novel serological assays and vaccines. Here, phage-peptide library panning coupled with screening using next generation sequencing was used to map antibody responses to bacterial infections. In the first instance, pigs experimentally infected with Salmonella enterica serovar Typhimurium was investigated. IgG samples from twelve infected pigs were probed in parallel and phage binding compared to that with equivalent IgG from pre-infected animals. Seventy-seven peptide mimotopes were enriched specifically against sera from multiple infected animals. Twenty-seven of these peptides were tested in ELISA and twenty-two were highly discriminatory for sera taken from pigs post-infection (P < 0.05) indicating that these peptides are mimicking epitopes from the bacteria. In order to further test this methodology, it was applied to differentiate antibody responses in poultry to infections with distinct serovars of Salmonella enterica. Twenty-seven peptides were identified as being enriched specifically against IgY from multiple animals infected with S. Enteritidis compared to those infected with S. Hadar. Nine of fifteen peptides tested in ELISA were highly discriminatory for IgY following S. Enteritidis infection (p < 0.05) compared to infections with S. Hadar or S. Typhimurium.

PMID:
 
27072017
 
PMCID:
 
PMC4829855
 
DOI:
 
10.1038/srep24232
[Indexed for MEDLINE] 
Free PMC Article
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