da Silva Raposo R1, Santarém VA2, Merigueti YF1, Rubinsky-Elefant G3, de Lima Cerazo LM4, Pereira L4, Zampieri BP4, da Silva AV5, Laposy CB6. 2016. Exp Parasitol. 171:33-41. doi: 10.1016/j.exppara.2016.09.009. Epub 2016 Oct 13.
Toxocariasis is a geohelminth zoonosis with worldwide distribution, mainly transmitted through the ingestion of embryonated eggs of nematodes of the Toxocara genus. The disease can also be transmitted to humans as a result of eating raw or undercooked meat of paratenic hosts, such as chickens. Here, we standardized an enzyme-linked immunosorbent assay (ELISA) for evaluating experimentally the kinetic and avidity index (AI) of IgY in broiler chickens infected with different doses of Toxocara canis eggs (G1:100; G2: 1000; and G3: 5000; n = 12 per group). The test showed 91.7% sensitivity (CI 95%: 77.5-98.3) and 100% specificity (CI 95%: 92.6-100), and highest efficiency (97.0%) at 60 days post infection. Infection was characterized by the presence of high avidity antibodies in the chronic phase. Our results support that the ELISA can be a highly useful tool for the detection of anti-Toxocara antibodies in chickens.
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Birds; ELISA; Immune response; Toxocariasis