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Kinetic and avidity of IgY anti-Toxocara antibodies in experimentally infected chickens.

Posted by on in 2016
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da Silva Raposo R1Santarém VA2Merigueti YF1Rubinsky-Elefant G3de Lima Cerazo LM4Pereira L4Zampieri BP4da Silva AV5Laposy CB6. 2016. Exp Parasitol. 171:33-41. doi: 10.1016/j.exppara.2016.09.009. Epub 2016 Oct 13. 

1Post-Graduation Program in Animal Science, Laboratory of Veterinary Parasitology, Veterinary Teaching-Hospital (UNOESTE), Presidente Prudente, São Paulo, Brazil.
2Laboratory of Veterinary Parasitology, Veterinary Teaching-Hospital, Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, São Paulo, Brazil. Electronic address: vamilton@unoeste.br.
3Laboratory of Seroepidemiology and Immunobiology, Instituto de Medicina Tropical de São Paulo, University of São Paulo, Brazil.
4Laboratory of Veterinary Parasitology, Veterinary Teaching-Hospital, Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, São Paulo, Brazil.
5Zoonosis and Public Health Research Group, State University of Feira de Santana, UEFS, Feira de Santana, Bahia, Brazil.
6Laboratory of Veterinary Clinical Pathology, Veterinary Teaching-Hospital, Universidade do Oeste Paulista (UNOESTE), Presidente Prudente, São Paulo, Brazil.

Abstract

Toxocariasis is a geohelminth zoonosis with worldwide distribution, mainly transmitted through the ingestion of embryonated eggs of nematodes of the Toxocara genus. The disease can also be transmitted to humans as a result of eating raw or undercooked meat of paratenic hosts, such as chickens. Here, we standardized an enzyme-linked immunosorbent assay (ELISA) for evaluating experimentally the kinetic and avidity index (AI) of IgY in broiler chickens infected with different doses of Toxocara canis eggs (G1:100; G2: 1000; and G3: 5000; n = 12 per group). The test showed 91.7% sensitivity (CI 95%: 77.5-98.3) and 100% specificity (CI 95%: 92.6-100), and highest efficiency (97.0%) at 60 days post infection. Infection was characterized by the presence of high avidity antibodies in the chronic phase. Our results support that the ELISA can be a highly useful tool for the detection of anti-Toxocara antibodies in chickens.

KEYWORDS:

Birds; ELISA; Immune response; Toxocariasis

PMID:
 
27746165
 
DOI:
 
10.1016/j.exppara.2016.09.009
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