Standing out in the field of IgY Immunotechnology

  • Home
    Home A full collection of all the Research Archive entries.
  • Years
    Years Sort entries by year.
  • Tags
    Tags Displays a list of tags that have been used in the blog.
  • Archives
    Archives Contains a list of research entries that were created previously.

Involvement of damage-associated molecular patterns in tumor response to photodynamic therapy: surface expression of calreticulin and high-mobility group box-1 release.*

Posted by on in 2011
  • Font size: Larger Smaller
  • Hits: 1713
  • Print
Korbelik MZhang WMerchant S., Cancer Immunol Immunother. 2011 Jun 5. [Epub ahead of print

British Columbia Cancer Research Centre, British Columbia Cancer Agency, 675 West 10th Avenue, Room 6.112, Vancouver, BC, V5Z 1L3, Canada, mkorbelik@bccrc.ca.

Abstract

Damage-associated molecular patterns (DAMPs), danger signal molecules expressed after injury or infection, have become recognized as prerequisite for orchestrating effective anti-tumor host response. The expression of two prototypical DAMPs, calreticulin and high-mobility group box-1 (HMGB1) protein, was examined following Photofrin™-photodynamic therapy (PDT) of Lewis lung carcinoma (LLC) cells in vitro and LLC tumors growing in syngeneic mice. Cell surface expression of calreticulin was found to be highly increased at 1 h after PDT treatment both in vitro and in vivo. Increased exposure of calreticulin was also detected on the surface of macrophages from PDT-treated LLC tumors. At the same time interval, a rise in serum HMGB1 was detected in host mice. Intracellular staining of macrophages co-incubated for 16 h with PDT-treated LLC cells revealed elevated levels of HMGB1 in these cells. The knowledge of the involvement of these DAMPs uncovers important mechanistic insights into the development of host response induced by PDT.

*Note: The FITC-labeled Donkey anti-IgY used in this publication was manufactured by Gallus Immunotech Inc.

 

Last modified on