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Interest of major serum protein removal for Surface-Enhanced Laser Desorption/Ionization - Time Of Flight (SELDI-TOF) proteomic blood profiling

Posted by on in 2006
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 Stéphane Roche (1)(2) Laurent Tiers (1) Monique Provansal (1)(3) Marie-Thérèse Piva (2)(3) and Sylvain Lehmann (1)(2)(3)

Proteome Sci. 2006; 4: 20. Published online 2006 October 5. doi: 10.1186/1477-5956-4-20.
Copyright © 2006 Roche et al; licensee BioMed Central Ltd.
Received June 20, 2006; Accepted October 5, 2006.

Abstract
Background

Surface-Enhanced Laser Desorption/Ionization - Time Of Flight (SELDI-TOF) has been proposed as new approach for blood biomarker discovery. However, results obtained so far have been often disappointing as this technique still has difficulties to detect low-abundant plasma and serum proteins.
Results

We used a serum depletion scheme using chicken antibodies against various abundant proteins to realized a pre-fractionation of serum prior to SELDI-TOF profiling. Depletion of major serum proteins by immunocapture was confirmed by 1D and 2D gel electrophoresis. SELDI-TOF analysis of bound and unbound (depleted) serum fractions revealed that this approach allows the detection of new low abundant protein peaks with satisfactory reproducibility.
Conclusion

The combination of immunocapture and SELDI-TOF analysis opens new avenues into proteomic profiling for the discovery of blood biomarkers.

1 Institut de Génétique Humaine du CNRS, 141 rue de la Cardonille, 34396 Montpellier, France
2 CHU Montpellier, Laboratoire de Biochimie, Hôpital St. Eloi, 80, av A. Fliche, 34295 Montpellier Cedex 5, France
3 Faculté de Médecine Montpellier - Nîmes, UM1 - UFR Médecine, 2, rue École de Médecine, 34060 Montpellier, France

Corresponding Author:
Stéphane Roche: stephane.roche@igh.cnrs.fr;
Laurent Tiers: laurent.tiers@igh.cnrs.fr;
Monique Provansal: monique.provansal@univ-montp1.fr;
Marie-Thérèse Piva: piva@univ-montp1.fr;
Sylvain Lehmann: sylvain.lehmann@igh.cnrs.fr

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