Standing out in the field of IgY Immunotechnology

  • Home
    Home A full collection of all the Research Archive entries.
  • Years
    Years Sort entries by year.
  • Tags
    Tags Displays a list of tags that have been used in the blog.
  • Archives
    Archives Contains a list of research entries that were created previously.

Immunologically active peptides that accompany hen egg yolk immunoglobulin Y: separation and identification.

Posted by on in 2013
  • Font size: Larger Smaller
  • Hits: 1597
  • Print

Polanowski A1Sosnowska AZabłocka AJanusz MTrziszka T. 2013. Biol Chem. 394:879-87. doi: 10.1515/hsz-2012-0337.

1Faculty of Food Sciences, Wroc ł aw University of Environmental and Life Sciences, ul. Chełmońskiego 37, 51-630 Wrocław, Poland. antonipolanowski@gmail.com

Abstract

The protein mixture of cytokine-inducing activity accompanying chicken immunoglobulin Y, named yolkin, consists of several peptides of molecular weight (MW) ranging from over 1 to 35 kDa. Yolkin and its constituent peptides were found to be efficient inducers of interleukin (IL)-1β, IL-6 and IL-10 secretion. N-terminal amino acid sequences of eight of the electrophoretically purified yolkin constituents revealed that all of them are homological to some fragments of the C-terminal domain of vitellogenin II. The fractions of MW about 4 and 12 kDa are free of carbohydrates and start at position 1732 in the vitellogenin amino acid sequence; whereas the other fractions (MW about 16, 19, 23, 29, 32 and 35 kDa) appeared to be glycoproteins corresponding to the amino acid sequence of vitellogenin starting at position 1572. From these data, it is concluded that yolkin most likely represents vitellogenin-derived peptides that possess cytokine-inducing activity and are, at least partially, responsible for such properties of separated immunoglobulin Y preparation. This finding reveals a new role for vitellogenin as a reservoir of polypeptides that may play an important role in the innate immune system of the developing embryo.

PMID:
23492558
[PubMed - indexed for MEDLINE]
Last modified on