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IgY14 and SuperMix immunoaffinity separations coupled with liquid chromatography-mass spectrometry for human plasma proteomics biomarker discovery.

Posted by on in 2012
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Shi TZhou JYGritsenko MAHossain MCamp DG 2ndSmith RDQian WJ. 2012. Methods. 56:246-53. doi: 10.1016/j.ymeth.2011.09.001. Epub 2011 Sep 10.

Biological Sciences Division and Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory, Richland, WA 99352, USA.

Abstract

Interest in the application of advanced proteomics technologies to human blood plasma- or serum-based clinical samples for the purpose of discovering disease biomarkers continues to grow; however, the enormous dynamic range of protein concentrations in these types of samples (often >10 orders of magnitude) represents a significant analytical challenge, particularly for detecting low-abundance candidate biomarkers. In response, immunoaffinity separation methods for depleting multiple high- and moderate-abundance proteins have become key tools for enriching low-abundance proteins and enhancing detection of these proteins in plasma proteomics. Herein, we describe IgY14 and tandem IgY14-Supermix separation methods for removing 14 high-abundance and up to 60 moderate-abundance proteins, respectively, from human blood plasma and highlight their utility when combined with liquid chromatography-tandem mass spectrometry for interrogating the human plasma proteome.

Copyright © 2011 Elsevier Inc. All rights reserved.

PMID:
21925605
[PubMed - indexed for MEDLINE]
PMCID:
PMC3261331
[Available on 2013/2/1]
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