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Fluorescence polarization immunoassay using IgY antibodies for detection of valnemulin in swine tissue.

Posted by on in 2015
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Zhang H1Mi T1Khan OY2Sheng Y1Eremin SA2Beier RC3Zhang S1Shen J1Wang Z4. 2015. Anal Bioanal Chem. 407(25):7843-8. doi: 10.1007/s00216-015-8948-z. Epub 2015 Aug 16.

  • 1College of Veterinary Medicine, China Agricultural University, State Key Laboratory of Agrobiotechnology, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing, 100193, China.
  • 2Department of Chemical Enzymology, Faculty of Chemistry, M. V. Lomonosov Moscow State University, Moscow, 119992, Russia.
  • 3U.S. Department of Agriculture, Agricultural Research Service, Southern Plains Agricultural Research Center, Food and Feed Safety Research Unit, College Station, TX, 77845, USA.
  • 4College of Veterinary Medicine, China Agricultural University, State Key Laboratory of Agrobiotechnology, Beijing Laboratory for Food Quality and Safety, Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing, 100193, China. wangzhanhui@cau.edu.cn.

Abstract

Immunoglobulin Y (IgY) is derived from egg yolk and has been identified as a cheap and high-yield immunoreagent. The application of IgY in immunoassays for the detection of chemical contaminants in food samples has rarely been reported. In this work, Three fluorophore-labeled VAL tracers were synthesized and the sensitivity of the best tracer (VAL-DTAF) in the optimized FPIA with antibody IgY100 demonstrated an IC50 value of 12 ng mL(-1) in buffer. After evaluation of several extraction procedures, acidified acetonitrile was selected to extract VAL from swine tissue. The recoveries of VAL in spiked swine tissue at three levels (50, 100, and 200 μg kg(-1)) were higher than 79% with coefficients of variation (CVs) lower than 12%. The limit of detection (LOD) of the FPIA in swine tissue was 26 μg kg(-1) and was lower than the maximum residue limit (MRL) of VAL set by the European Union. The study showed that IgY could be a good substitute for IgG when developing a high-throughput assay for chemical residues.

KEYWORDS:

Chemical residues; Fluorescence polarization immunoassay; IgY; Swine tissue; Valnemulin

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