Standing out in the field of IgY Immunotechnology

  • Home
    Home A full collection of all the Research Archive entries.
  • Years
    Years Sort entries by year.
  • Tags
    Tags Displays a list of tags that have been used in the blog.
  • Archives
    Archives Contains a list of research entries that were created previously.

Expression of heavy chain-only antibodies can support B-cell development in light chain knockout chickens.

Posted by on in 2016
  • Font size: Larger Smaller
  • Hits: 900
  • Print

Schusser B1Collarini EJ2Pedersen D2Yi H2Ching K2Izquierdo S2Thoma T1Lettmann S3Kaspers B3Etches RJ2van de Lavoir MC2Harriman W2Leighton PA42016. Eur J Immunol. 46(9):2137-48. doi: 10.1002/eji.201546171. Epub 2016 Aug 2.

1Reproductive Biotechnology, Technische Universität München, WZW Center of Life Science, Freising-Weihenstephan, Germany.
2Crystal Bioscience Inc, Emeryville, CA, USA.
3Department of Veterinary Science, Institute for Animal Physiology, Ludwig-Maximilians-Universitaet Muenchen, Munich, Germany.
4Crystal Bioscience Inc, Emeryville, CA, USA. pal@crystalbioscience.com.

 

Abstract

Since the discovery of antibody-producing B cells in chickens six decades ago, chickens have been a model for B-cell development in gut-associated lymphoid tissue species. Here we describe targeting of the immunoglobulin light chain locus by homologous recombination in chicken primordial germ cells (PGCs) and generation of VJCL knockout chickens. In contrast to immunoglobulin heavy chain knockout chickens, which completely lack mature B cells, homozygous light chain knockout (IgL(-/-) ) chickens have a small population of B lineage cells that develop in the bursa and migrate to the periphery. This population of B cells expresses the immunoglobulin heavy chain molecule on the cell surface. Soluble heavy-chain-only IgM and IgY proteins of reduced molecular weight were detectable in plasma in 4-week-old IgL(-/-) chickens, and antigen-specific IgM and IgY heavy chain proteins were produced in response to immunization. Circulating heavy-chain-only IgM showed a deletion of the CH1 domain of the constant region enabling the immunoglobulin heavy chain to be secreted in the absence of the light chain. Our data suggest that the heavy chain by itself is enough to support all the important steps in B-cell development in a gut-associated lymphoid tissue species.

KEYWORDS:

Antibodies; B-cell development; Immunoglobulins; Knockout chickens

PMID:
 
27392810
 
PMCID:
 
PMC5113765
 
DOI:
 
10.1002/eji.201546171
Last modified on