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Evaluation of Chicken IgY Generated Against Canine Parvovirus Viral-Like Particles and Development of Enzyme-Linked Immunosorbent Assay and Immunochromatographic Assay for Canine Parvovirus Detection.

Posted by on in 2015
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He J1Wang Y1Sun S2Zhang X1. 2015. Viral Immunol. 28(9):489-94. doi: 10.1089/vim.2015.0030. Epub 2015 Oct 15.

  • 11 College of Veterinary Medicine, Northwest A&F University , Yangling, Shaanxi, People's Republic of China .
  • 22 Key Laboratory of Animal Virology of Ministry of Agriculture, Lanzhou Veterinary Research Institute , Chinese Academy of Agricultural Sciences, Lanzhou, China .

Abstract

Immunoglobulin Y (IgY) antibodies were generated against canine parvovirus virus-like particles (CPV-VLPs) antigen using chickens. Anti-CPV-VLPs-IgY was extracted from hen egg yolk and used for developing enzyme-linked immunosorbent assay (ELISA) and immunochromatographic assay (ICA) for the detection of CPV in dog feces. The cutoff negative values for anti-CPV-VLPs-IgY were determined using negative fecal samples (already confirmed by polymerase chain reaction [PCR]). In both ELISA and ICA, there was no cross-reaction with other diarrheal pathogens. Thirty-four fecal samples were collected from dogs with diarrhea, of which 26.47% were confirmed as CPV-positive samples by PCR, while 29.41% and 32.35% of the samples were found to be positive by ELISA and ICA, respectively. The developed ELISA and ICA exhibited 97.06% and 94.12% conformity with PCR. Higher sensitivity and specificity were observed for IgY-based ELISA and ICA. Thus, they could be suitable for routine use in the diagnosis of CPV in dogs.

PMID:
 
26469376
 
[PubMed - in process]
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