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Evaluation and optimization of IgY Spin Column technology in the depletion of abundant proteins from human serum.

Posted by on in 2011
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Corrigan LJefferies CClive Lee TDaly J., 2011, Proteomics. 11(16):3415-9

Division of Biology, Royal College of Surgeons in Ireland, Dublin, Ireland; Department of Anatomy, Royal College of Surgeons in Ireland, Dublin, Ireland.


Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin columns. However, modification of the manufacturer's protocol, subjecting samples to two rounds of depletion, improved the number of proteins resolved by 2-DE. With alteration of the manufacturer protocol, the Seppro(®) IgY14 spin column can produce depleted serum with an increased number of spots resolved by 2-DE compared to untreated serum.

Copyright © 2011 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

[PubMed - in process]


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