Standing out in the field of IgY Immunotechnology

  • Home
    Home A full collection of all the Research Archive entries.
  • Years
    Years Sort entries by year.
  • Tags
    Tags Displays a list of tags that have been used in the blog.
  • Archives
    Archives Contains a list of research entries that were created previously.

Egg yolk antibodies against the E7 oncogenic protein of human papillomavirus type 16.

Posted by on in 2001
  • Font size: Larger Smaller
  • Hits: 1695
  • Print

 Di Lonardo AD, Marcante ML, Poggiali F, Hamsøíkovà E, Venuti A
Arch Virol 2001 146:117-25

Abstract


The E7 oncoprotein is the major transforming protein of Human Papillomavirus type 16 (HPV16) and the most abundant in cervical neoplasia. In this study we report the production of polyclonal antibodies to HPV16 E7 in rabbits and hens. The produced antibodies recognised the denatured and native form of HPV16 E7 protein by Western Blot, and immunoprecipitation. Epitope mapping demonstrated that hen antibodies reacted with a greater number of antigen determinants than the rabbit antibodies. In immunocytochemistry only hen antibodies were able to localize the E7 protein in a HPV positive cell line and in the high-grade squamous intraepithelial lesions, suggesting their possible usefulness in the screening of clinical samples.

Author Address
Laboratory of Virology, Regina Elena Institute for Cancer Research, Rome, Italy

Last modified on