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Efficacy of specific IgY for treatment of lipopolysaccharide-induced endotoxemia using a mouse model.

Posted by on in 2011
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Zhen YHFang RDing CJin LJLi XYDiao YPShu XHMa XCXu YP. 2011. J Appl Microbiol. 111:1524-32. doi: 10.1111/j.1365-2672.2011.05155.x. Epub 2011 Oct 6.

College of Pharmacy, Dalian Medical University, Dalian, China.

Abstract

AIMS:

To estimate the efficacy of specific egg yolk immunoglobulin (IgY) for the treatment of lipopolysaccharide (LPS)-induced endotoxemia using a mouse model.

METHODS AND RESULTS:

Specific IgY was obtained from the yolk of hens immunized with formaldehyde-killed Escherichia coli O111 and showed a high binding activity to LPS when subjected to an ELISA. Endotoxemia was induced in mice by intraperitoneal injection of LPS at a dose of 20 mg kg(-1) for measuring survival rate and 10 mg kg(-1) for cytokine measurement. The survival rate of mice treated with 200 mg kg(-1) specific IgY or 5 mg kg(-1) dexamethasone was 70% while none of the mice in the normal saline-treated group survived more than 7 days. Specific IgY significantly (P < 0.05) decreased tumour necrosis factor-α (TNF-α) level and increased interleukin-10 (IL-10) level in the serum of endotoxemia mice. Specific IgY had less of an effect on TNF-α than dexamethasone, while its effect on increasing IL-10 was stronger than dexamethasone. Haematoxylin and eosin-stained sections indicated that IgY attenuated the damage to the lung and liver observed in mice with endotoxemia.

CONCLUSIONS:

The specific IgY increased the survival rate of mice with endotoxemia induced by LPS, down-regulated TNF-α and up-regulated IL-10 in serum and attenuated the extent of damage to the lung and liver.

SIGNIFICANCE AND IMPACT OF THE STUDY:

The specific IgY has potential for the treatment of LPS-induced endotoxemia.

© 2011 The Authors. Journal of Applied Microbiology © 2011 The Society for Applied Microbiology.

PMID:
21933310
[PubMed - in process]
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