Department of Parasitology, Medical College, Zhengzhou University, 40 Daxue Road, Zhengzhou 450052, PR China.
In this study, a sandwich ELISA based on IgY (egg yolk immunoglobulin) and IgM monoclonal antibody (mAb) against excretory-secretory (ES) antigens of Trichinella spiralis muscle larvae was developed for detection of circulating antigens (CAg) in serum from mice infected with T. spiralis. The IgY-IgM sandwich ELISA involved the use of chicken antibody IgY as a capture antibody and mouse IgM mAb 5A7G4 as a detecting antibody. This assay was able to detect as little as 1ng/ml of ES antigens added to normal mouse serum. Two groups of BALB/c mice infected with T. spiralis larvae were used: heavily infected mice (20 mice infected with 300 larvae) and lightly infected mice (20 mice infected with 100 larvae) and 10 normal mice as control. The CAg was detectable as early as 4days post infection (dpi) in the sera from both groups of infected mice, then increased rapidly, reached a peak with detection rate of 100% in heavily infected mice at 10dpi and 80% in lightly infected mice at 22dpi, respectively. The anti-Trichinella IgM antibodies was first detected in 40% of heavily infected mice and 20% of lightly infected mice at 8dpi, and reached a peak positive rate of 100% in heavily infected mice at 16dpi and in lightly infected mice at 26dpi, respectively. The novel assay appears to be sensitive for detection of antigens of T. spiralis and valuable to the early diagnosis of trichinellosis.
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