[Circular dichroism chromatography of secondary structures of anti-HBV IgY in eggs from hens immunized with recombinant yeast HBV vaccine].

Fang X¹, Fang Y, Wang D, Qi F. 2013. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 29:1205-7.

Abstract

OBJECTIVE:
To study the generation and structure of anti-HBV IgY in eggs from hens immunized with recombinant yeast HBV vaccine.

METHODS:
From the hens immunized three times with the recombinant yeast HBV vaccine, the eggs were collected to extract anti-HBV IgY. After purified, the anti- HBV IgY was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and reverse phase high-performance liquid chromatography (RP-HPLC), and its secondary structure was examined by circular dichroism (CD) chromatography.

RESULTS:
The relative molecular mass (Mr;) of anti-HBV IgY expressed by the recombinant yeast HBV vaccine on hens was 178 000. CD chromatography showed that the α-helix, β-fold, β-turn and random coil of anti-HBV IgY had 38, 0, 44 and 17 amino acids, respectively.

CONCLUSION:
The anti-HBV IgY can be expressed in eggs from hens immunized with recombinant yeast HBV vaccine. The α-helix and β-turn are major secondary structures of anti-HBV IgY.

PMID: