Standing out in the field of IgY Immunotechnology

  • Home
    Home A full collection of all the Research Archive entries.
  • Years
    Years Sort entries by year.
  • Tags
    Tags Displays a list of tags that have been used in the blog.
  • Archives
    Archives Contains a list of research entries that were created previously.

CART (cocaine- and amphetamine-regulated transcript) peptide receptors: specific binding in AtT20 cells.*

Posted by on in 2005
  • Font size: Larger Smaller
  • Hits: 2941
  • Print

Vicentic A, Lakatos A, Kuhar MJ., 2005, Eur J Pharmacol. 528(1-3):188-9.

Neuroscience Division, Yerkes National Primate Research Center of Emory University, 954 North Gatewood Road, Atlanta Georgia 30033, USA.


Given previous evidence for CART (cocaine- and amphetamine-regulated transcript) signaling in AtT20 cells, the binding of [125I]-CART61-102 was characterized in these cells. The binding was specific, saturable, dependent on time, pH, temperature and protein concentration, with a Bmax of 101.4+/-8.8 fmol/mg protein and a Kd of 21.9+/-8.0 pM. Only active CART55-102, but not other peptides or drugs, inhibited the [125I]-CART61-102 binding. These data are the first demonstration of specific receptor binding for CART peptides.

*Custom peptides and IgY antibodies used in this publication were produced by Gallus Immunotech Inc. Please visit our Custom Peptide Synthesis and Custom IgY production pages for more information.

Last modified on