Jacques Robert, , Jennifer Gantress, Nicholas Cohen and Gregory D. Maniero
Department of Microbiology and Immunology, University of Rochester Medical Center, Rochester, NY 14642, USA
Methods, 2004, 32: 42-53 Heat Shock Proteins and Immune Response
Abstract
The frog Xenopus provides a unique model system for studying the evolutionary conservation of the immunological properties of heat shock proteins (hsps). General methods for maintaining and immunizing isogenetic clones of defined MHC genotypes are presented together with more recently developed protocols for exploring hsp-mediated immune responses in vitro (proliferative and cytotoxic assays) and in vivo (adoptive cell transfer and antibody treatment) in adults and in naturally MHC class I-deficient larvae. Finally, techniques to study modalities of expression of the endoplasmic reticulum resident gp96 at the cell surface of tumor and normal lymphocytes are considered.
Author Keywords: Xenopus; Proliferative assay; Cytotoxicity; CFSE; Heat shock protein; gp96; NK cells; CD8 T cells; Phylogeny; Evolution
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