Brooke D. Humphrey , Chris C. Calvert and Kirk C. Klasing ,
Department of Animal Science, University of California, One Shields Avenue, Davis, CA 95616, USA
Developmental & Comparative Immunology, 2004, 28: 665-672
Abstract
Ducks produce a full length IgY and a truncated isoform (IgYDFc). IgY and IgY(DFc) were isolated from ducks vaccinated against Escherichia coli and used to make E.coli-Ig immune complexes (IC). Phagocytosis of IC by duck monocytes decreased directly with the proportion of IgYDFc (p<0.001). IC containing IgY:IgY(DFc) at ratios of 100:0, 50:50, 0:100, and 0:0 (E. coli alone) were injected intravenously into naïve mallard ducks. At 24 h after injection, plasma hemopexin levels were higher in ducks given either the 0:100 ratio or the 100:0 ratio than those given 50:50 or E. coli alone (p<0.05). Liver IL-1 mRNA levels followed a similar pattern. Splenic IL-1 mRNA decreased markedly as the proportion of IgY(DFc) increased (p<0.01). Thus, IgY(DFc) may shift the response to IC from the spleen to the liver as infections progress from acute to chronic.
Author Keywords: Duck; IgY; IgY(DFc); Acute phase response; Immune complex; Phagocytosis; Interleukin-1; Hemopexin C¢, complement; FcR, IgG Fc-receptor; IC, immune complex; Ig, immunoglobulin; PBM, peripheral blood monocyte; s.q., subcutaneous; IgY(DFc), truncated IgY
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