Nikoo Arasteh (a), A. -H. Aminirissehei (b), A. N. Yousif (a), L. J. Albright (b) and T. D. Durance (a)
Aquaculture, 2004, 231: 23-36
Abstract
Passive immunization using pathogen-specific antibodies raised in chickens is a potential method for the protection of teleosts against diseases. The oral delivery route offers technical and economic advantages. In this study, the water-soluble fraction (WSF) of the egg yolks of vaccinated hens was used as a source of anti-Vibrio anguillarum IgY in intraperitoneal (IP) injection, oral intubation, or feeding of rainbow trout. IP-injected anti-Vibrio IgY was transferred into the trout system in high-enough levels to confer protection against vibriosis in an experimental challenge. This protective effect was retained at least 14 days post IgY injection and proved the efficacy of pathogen-specific IgY in enhancement of disease resistance. Oral intubation and feeding of anti-Vibrio IgY with sodium bicarbonate and Mega9, deoxycholate, or octyl--glucoside (OBG) resulted in different levels of protection against vibriosis and, in some cases, were comparable to the protection offered by IP injection of IgY.
Author Keywords: Egg yolk immunoglobulins (IgY); Vibrio anguillarum; Rainbow trout; Oral administration; Challenge
Corresponding author. Tel.: +1-604-822-2544; fax: +1-604-822-3959
(a) Food, Nutrition and Health, The University of British Columbia, Food Science Building, 6650 Northwest Marine Drive, Vancouver, British Columbia, Canada V6T 1Z4
(b) Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, British Columbia, Canada V5A 1S6 | 
