D. Jönsson (a), (b), G. Andersson (b), E. Ekblad (a), M. Liang (a), G. Bratthall (b) and B. -O. Nilsson (a)
Archives of Oral Biology, 2004, 49: 85-88
Abstract
Two transcription associated estrogen receptor (ER) subtypes have been identified and named ER and ER. In the present study we investigate the expression of these ER subtypes in cultured human periodontal ligament (PDL) cells by immunocytochemistry. ER immunoreactivity was observed in the nuclei of about 40% of the PDL cells, while no ER immunoreactivity was detected. In human breast cancer MCF-7 cells, serving as positive controls, both ER and ER immunoreactivities were demonstrated. No immunoreactivity was observed after omission of the primary antibodies. This study suggests that estrogen acts on gene transcription preferentially via ER in human PDL cells.
Author Keywords: Estrogen receptors; Immunocytochemistry; Periodontal ligament cells
Corresponding author. Tel.: +46-46-2227769; fax: +46-46-2224546.
(a) Department of Physiological Sciences, Section for Molecular and Cellular Physiology, Lund University, BMC F12, S-221 84, Lund, Sweden
(b) Department of Periodontology, Odontological Faculty, Malmö University, S-214 21, Malmo, Sweden | 
