IgY EggsPress Purification Kit
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Our IgY EggsPress Purification Kit enables researchers to quickly and efficiently purify chicken antibodies (IgY) from the eggs of their immunized chickens without loss of activity. There are many advantages in choosing chickens to raise your polyclonal antibodies.
The disadvantage of using hens is the difficulty of purifying the IgY from the lipid environment of the egg yolk. This problem is circumvented by using our IgY antibody purification kit. Two precipitation steps — one to remove the lipid and the second to precipitate the IgY — provide you with a 90% pure IgY fraction. Expect to purify between 4 and 7 mg IgY per ml egg yolk.
Simply follow the antibody purification Kit Instructions. The entire procedure takes about 4 hours but only 30 minutes is actual hands-on time.
Once you have your IgY, you can treat it like any immunoglobulin. You may wish to label or affinity-purify your antibody. Proceed as you would with any antibody fraction. Keep in mind that IgY does not bind to protein A, protein G, or protein L! Perhaps you want to use your IgY as a primary antibody in an ELISA test, Western blot or immunostaining procedure. A selection of labeled secondary reagents (anti-chicken immunoglobulins) and normal (pre-immune) IgY are available.
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The answer is simple.
Compared to the competition, not only is our chicken antibody purification kit cheaper to buy (per mg IgY purified) but the IgY yield obtained per gram egg yolk is, on average, 50% higher.
Unlike other IgY purification kits, our kit is polyethylene glycol (PEG) - free. High molecular weight PEG may interfere with your antibody assay and is difficult to remove.
Our antibody purification kit comes with detailed instructions and technical support is always available. Email us with any questions you may have.
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5 egg yolk isolations (Cat. No. IK 500)
- Reagent A (500 ml)
- Reagent B (500 ml)
- Egg Separator
- Instructions
20 egg yolk isolations (Cat. No. IK 2000)
- Reagent A (4 x 500 ml)
- Reagent B (4 x 500 ml)
- Egg Separator
- Instructions
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- Keep at room temperature until ready to use.
- Reagents A and B must be refrigerated before use.
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- Egg yolks
- Distilled Water
- Phosphate-buffered saline (PBS)
- Pasteur pipette
- Balance
- Beaker, graduated cylinder
- Stir plate and stir bar
- Refrigerator
- Centrifuge
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Perhaps a 90% pure IgY preparation is not necessary for your experimental procedure. Delipidation of the egg yolk provides the researcher with a diluted IgY solution (also containing other yolk proteins) which, concentrated and dialyzed, can be used for many applications. To delipidate your egg yolk only, egg separators and Reagent A are available separately (see below).
Reagent B, our IgY precipitation reagent, is also available separately to purify IgY from other yolk proteins after delipidation.
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Our Guarantee
We guarantee that if you follow the kit Kit Instructions and if you do not obtain at least 4 mg of 90% pure IgY per ml yolk, we will refund the cost of the kit.
Don't want to purify your IgY yourself?
Ask us to perform your chicken IgY purification for you >> Antibody purification
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