Empey Campora C, Hokama Y, Yabusaki K, Isobe M., 2008, J Clin Lab Anal.:22(4):239-45.
Department of Pathology, John A. Burns School of Medicine, Honolulu, Hawaii 96822, USA. empey@hawaii.edu
A sandwich enzyme-linked immunosorbent assay was developed to detect ciguatoxin (CTX) in fish tissue. The assay utilizes two antibodies, chicken immunoglobulin Y specific to the ABCD domain of CTX and a mouse monoclonal immunoglobulin G-horseradish peroxidase conjugate specific to the JKLM domain of CTX. The sensitivity, working range, cross reactivity, accuracy, precision, and reproducibility were examined. |