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Development of an enzyme-linked immunosorbent assay for the detection of ciguatoxin in fish tissue using chicken immunoglobulin Y.

Empey Campora C, Hokama Y, Yabusaki K, Isobe M., 2008, J Clin Lab Anal.:22(4):239-45.

Department of Pathology, John A. Burns School of Medicine, Honolulu, Hawaii 96822, USA. empey@hawaii.edu

A sandwich enzyme-linked immunosorbent assay was developed to detect ciguatoxin (CTX) in fish tissue. The assay utilizes two antibodies, chicken immunoglobulin Y specific to the ABCD domain of CTX and a mouse monoclonal immunoglobulin G-horseradish peroxidase conjugate specific to the JKLM domain of CTX. The sensitivity, working range, cross reactivity, accuracy, precision, and reproducibility were examined.

 
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