Motoi Y, Inoue S, Hatta H, Sato K, Morimoto K, Yamada A.
Jpn J Infect Dis. 2005 Apr;58(2):115-8.
The United Graduate School of Veterinary Science, Gifu University, Gifu 501-1193, Japan.
Abstract
We obtained rabies-specific egg yolk antibodies (IgY) by immunizing hens with recombinant His-tagged nucleoprotein and phosphoprotein (rN, rP) of the rabies virus (CVS-11 strain) expressed in Escherichia coli. The anti-rN and rP IgY were shown to bind specifically to the respective proteins of the CVS-11 strain of rabies virus by Western blotting, immune fluorescent assay and immunohistochemistry, indicating that IgY to rabies recombinant proteins could serve as a reagent for diagnosis of rabies virus infection. |