Hansson LO, Flodin M, Nilsen T, Caldwell K, Fromell K, Sunde K, Larsson A.
J Immunoassay Immunochem. 2008;29(1):1-9.
Department of Medical Sciences, University Hospital, Uppsala, Sweden.
We have compared three commercial particle enhanced cystatin C reagents. One of the reagents utilizes chicken antibodies and the other two reagents are rabbit antibody based. We show that the chicken antibody based reagent yields a higher delta absorbance when reacting with the antigen. IgY coupled to latex particles show a strong scatter response even at high antigen concentrations in contrast to the steep decline in scatter previously reported for IgY antibodies in solution. The reagent also showed a low CV for duplicate samples. Laying hens thus seems as an interesting source of antibodies for particle-enhanced immunoassays.
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