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AHA-HRP
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Anti-Hemagglutinin (HA)
Code:
AHA-HRP
$ 190.00 USD
Host:
Chicken
Volume:
0.2 mL
Quantity:
0.1 mg
Type:
HRP conjugated
Full Name:
HRP-Conjugated Affinity Purified Chicken Anti HA (Hemagglutinin) Epitope
Physical State:
liquid
Concentration:
0.5 mg/ml
Diluent:
25 mM HEPES pH 7.0, 0.05 M Sodium Chloride with 0.1% BSA, 50% glycerol
Preservative:
0.005% Thimerosal
Procedure:
Chickens were immunized with YPYDVPDYA (influenza hemagglutinin-HA- epitope) conjugated to KLH. After multiple immunizations in Freunds adjuvant, eggs were collected and the immunoglobulin fraction prepared. Antibodies were immunoaffinity purified using the peptide immobilized on a solid support and the antibodies were conjugated to horseradish peroxidase (HRP).
Specifications:
ELISA: The anti HA diluted 1:5,000 gave an O.D.= 1.0 in a 15 minute reaction against peptide conjugated to a different carrier than used for anti peptide purification. In Western Blot analyses, the chicken anti-HA was an effective probe at 1:500 dilution.
Storage:
4°C or frozen for long term. Do not freeze-thaw often.
Expiration Date:
0000-00-00
Note:
For in vitro diagnostic, research or proteomics applications only.
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