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Anti-Human
Osteopontin-c
Code: AhOPNc

$ 275.00 USD
Anti-Human<br>Osteopontin-c
Click to enlarge
Host: Chicken
Volume: 0.1 ml
Quantity: 0.1 mg
Type: Affinity purified
Full Name: Aff. Pur. Chicken anti-human osteopontin-c (the shortest splice variant of osteopontin)
Physical State: liquid
Concentration: 1.0 mg/ml
Diluent: Phosphate buffered saline, pH 7.2
Preservative: 0.05% Sodium Azide
Procedure: A chicken was immunized with a peptide representing the splice junction of osteopontin-c (Ac-SEEKQNAVSC-COOH). After multiple immunizations, eggs were collected and IgY was purified from the egg yolk. The antibody was affinity purified on an osteopontin-c peptide column.
Specifications: This antibody has been tested in ELISA and a suggested starting dilution for ELISA analysis is 2 ug/ml. Optimal working dilutions should be determined for your particular assay conditions. For Western blotting, recommended concentration 1 ug/ml.
Storage: 4°C
Expiration Date: 0000-00-00
Note: For in vitro diagnostic, research or proteomics applications only.
References: 1. He B, Mirza M, Weber GF., 2006. An osteopontin splice variant induces anchorage independence in human breast cancer cells. Oncogene 25:2192–2202.
2. Mirza M, Shaughnessy E, Hurley JK, Vanpatten KA, Pestano GA, He B, Weber GF., 2008. Osteopontin-c is a selective marker of breast cancer. Int J Cancer: 122:2646.
3. Sullivan J, Blair L, Alnajar A, Aziz T, Ng CY, Chipitsyna G, Gong Q, Witkiewicz A, Weber GF, Denhardt DT, Yeo CJ, Arafat HA., 2009. Expression of a prometastatic splice variant of osteopontin, OPNC, in human pancreatic ductal adenocarcinoma. Surgery 146:232-40.
4. Tilli, TM, Thuler LC, Matos, AR, Coutinho-Camillo, CM, Soares, FA, da Silva, EA, Neves, AF, Goulart, LR and ER Gimba. 2011.Expression analysis of osteopontin mRNA splice variants in prostate cancer and benign prostatic hyperplasia. Exp. Mol. Pathol. 92:13-19.