Anti-Bovine Serum
Albumin (BSA)
Code: ABSA-HRP

$ 85.00 USD



Host:	Chicken
Volume:	1.0 mL
Quantity:	0.5 mg
Type:	HRP conjugated
Full Name:	HRP-conjugated Chicken-anti-Bovine Serum Albumin (BSA)
Physical State:	liquid
Concentration:	0.5 mg/ml
Diluent:	25 mM HEPES pH 7.0, 0.05 M Sodium Chloride with 0.1% BSA, 50% Glycerol
Preservative:	0.005% thimerosal
Procedure:	Chickens were immunized with highly purified bovine serum albumin. After multiple immunizations in Freunds adjuvant, eggs were collected and the IgY fraction prepared. The antibodies were immunoaffinity purified from a solid phase immunoabsorbant and conjugated to horseradish peroxidase (HRP).
Specifications:	The anti BSA shows a single precipitin arc against bovine serum albumin and the albumin in bovine serum using immunoelectrophoresis. In a sandwich ELISA against purified bovine albumin with 20 ug/ml as sample, conjugate diluted 1: 3,000 produced an O.D.=1.2 in a 15 minute reaction with OPD as substrate. Optimal conjugate working dilutions should be determined for your particular assay conditions.
Storage:	4°C or frozen for long term. Do not freeze-thaw often.
Expiration Date:	0000-00-00
Note:	For research use only.