Meisel H
Biol Chem Hoppe Seyler 1994 Jun 375:401-5
Abstract
Antibodies (IgY) directed against a synthetic, bioactive peptide (beta-casokinin-10) were obtained from egg yolk of immunized chickens. Using a beta-casokinin-10/BSA conjugate for immunization, large quantities of high-titered anti-peptide antibodies were obtained. ELISA standard curves for beta-casokinin-10 were linear in the range 30-22,000 ng/ml. IgY-antibodies against beta-casokinin-10 recognized not only the immunogenic peptide structure but also analogues epitopes in protein preparations containing bovine beta- and alpha s-caseins, respectively, as well as in ovine caseins. The anti-beta-casokinin-10 IgY-antibodies are intended to be used as immunochemical reagents in future structure-activity studies of bioactive casokinins that are inhibitors of the angiotensin-converting enzyme.
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