Anti-Chicken IgA
(α chain specific)
Code: AChIgA-HRP

$ 130.00 USD



Host:	Goat
Volume:	1.0 ml
Quantity:	0.5 mg
Type:	HRP conjugated
Full Name:	Horseradish peroxidase-conjugated Affinity-Purified Goat Anti-Chicken IgA (alpha-chain)
Physical State:	liquid
Concentration:	0.5 mg/ml
Diluent:	25 mM HEPES pH 7.1, 0.05M sodium chloride with 0.1% BSA, 50%glycerol
Preservative:	0.005% Thimerosal
Procedure:	Goats were immunized with highly purified chicken IgA. The antiserum was solid phase absorbed to make IgA alpha chain specific and the anti-IgA antibodies were immunoaffinity purified and conjugated with horseradish peroxidase (HRP).
Specifications:	The affinity purified anti-IgA alpha reacts with IgA in serum and secretions. It does not react with IgM, IgY or light chains by IEP analysis. The conjugate, when tested in a sandwich ELISA against 1 ug chicken IgA as sample could be diluted 1:5000 to produce an O.D.=1.0 in a 30 minute reaction using TMB as substrate. Optimal working dilutions should be determined for your particular assay conditions.
Storage:	4°C, or -20°C for long-term storage. Do not freeze-thaw often.
Expiration Date:	0000-00-00
Note:	For research use only.
Reference:	1. van Ginkel, F.W., D. C. Tang, S. L. Gulley and H. Toro, 2009, Induction of mucosal immunity in the avian Harderian gland with a replication-deficient Ad5 vector expressing avian influenza H5 hemagglutinin. Dev. Comp. Immunol. 33: 28-34.